Millipore Sigma has a page dedicated to unit conversion, from temperature and pressure to flow rate and absorbance/transmittance. GraphPad’s website includes calculators for statistical analysis as well as chemical and radiochemical data. There’s no shortage of online calculators for a wide range of functions, including random number assignment, unit conversion, and buffer recipes. IDT also provides assistance on this subject, with options to select from predesigned sgRNAs, design custom sgRNAs, and assess those designs before ordering. Optimal sgRNA design is critical for success, and with that in mind, we have a curated list of web-based tools to help you in this endeavor, with features noted for each. The app is available for both iPhone and Android devices, and allows you to email the data to yourself for record keeping.ĭesigning a single guide RNA (sgRNA) to target your gene of interest is the first step in CRISPR/Cas9 gene editing. This quick protocol can replace lengthy traditional methods such as qPCR and ELISA for determining accurate viral titer. The Lenti-X GoStix app works in conjunction with Lenti-X GoStix Plus to quantify lentivirus in your viral prep, in just ten minutes. You can even manually edit and delete detected colonies. The EZ Colony Counter for iPhone makes use of your smartphone’s camera to capture images of your transformation plate, then detects and records colony number. The Double Digest Finder and Enzyme Finder are also available for iPhone and Android devices. NEB provides support for traditional cloning methods, with tools for planning restriction digests, calculating molar ratios for ligation, and viewing recommended guidelines for ligation protocols. This web tool accommodates single- or multiple-insert cloning, vector linearization by inverse PCR or restriction digest, and site-directed mutagenesis. Our cloning tools page supports seamless In-Fusion Cloning with a molar ratio calculator and step-by-step primer design tool. While the software can be used for traditional restriction digest methods, it also has built-in support for Gateway Cloning, Gibson Assembly, In-Fusion Cloning, and TA cloning. SnapGene Viewer lets you easily visualize your molecular biology procedures. Regardless of which cloning method you use, you’ll want a good way to plan your project. Determine reaction conditions and identify problematic sequences before you use up valuable reagents and templates. Primer design is critical for every PCR and qPCR assay, and IDT provides several web tools for designing, analyzing, and diluting your primers. This easy access to the internet means that you have a number of incredibly helpful research tools right at your fingertips.įrom cloning to virus prep, from PCR to CRISPR/Cas9 experiments, we’ve compiled a list of helpful resources to make your work easier. Even if you’re not in front of a computer, odds are you have your smartphone with you, even in the lab. Luckily, we live in an age of convenient digital solutions that can simplify experimental calculations and planning. Life in the lab can be fast-paced, with little time between incubations to prepare for the next stage of the experiment. Convenient tools to complement your labwork
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